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Study regeneration role and possible mechanism of metanephric mesenchymal and metanephric mesenchymal transfected by uPA gene in CKD.Method：1. Construction and package of recombinant plasmid of uPA (urokinase-type plasminogen activator) gene and pAAV-IRES- hrGFP vector.2. The viral particles of rAAV and AAV were transfected into rats metanephric mesenchymal (rat-inducible metanephric mesenchymal-18, RIMM-18) in vitro and the positive RIMM-18 cells that expressed GFP were detected using the inverted fluorescence microscope.3.Study regeneration role and possible mechanism of metanephric mesenchymal and metanephric mesenchymal transfected by uPA gene in CKD that were transplanted via caudal vein.Conclution：1.The recombinant plasmid of AAV and uPA gene were successfully constructed andpackaged. 2.The transfectian rate of rAAV and AAV viral particles to RIMM-18 was as high as 60％. AAV vectorcan deliver uPA gene effectively.