【摘 要】
：
为调查和研究贾第虫的流行性和基因型,使用SSU rRNA,tpi,gdh,和bg基因多位点分析方法,对采集的43个种类1,882份非人灵长类的粪便样品进行检测盒分析.结果显示,基于tpi基因,22个种类的122份(6.5％)非人灵长类的样本检测到贾第虫,包括恒河猴(2.7％),阿萨姆邦猕猴(11.1％),食蟹猴(10.0％),长尾猕猴(52.9％),日本猕猴(33.3％),白头叶猴(0.7％),赤
【机 构】
：
河南农业大学牧医工程学院,郑州 450002 郑州动物园,郑州 450002
【出 处】
：
中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会
论文部分内容阅读
为调查和研究贾第虫的流行性和基因型,使用SSU rRNA,tpi,gdh,和bg基因多位点分析方法,对采集的43个种类1,882份非人灵长类的粪便样品进行检测盒分析.结果显示,基于tpi基因,22个种类的122份(6.5％)非人灵长类的样本检测到贾第虫,包括恒河猴(2.7％),阿萨姆邦猕猴(11.1％),食蟹猴(10.0％),长尾猕猴(52.9％),日本猕猴(33.3％),白头叶猴(0.7％),赤猴(31.3％),白须猴(100％),叶猴(9.1％),金丝猴(13.2％),黑白疣猴(14.3％),白腹长尾猴(16.7％),松鼠猴(22.2％),绿猴(17.7％),懒猴(50.0％),环尾狐猴(56.5％),山魈(5.1％),阿拉伯狒狒(3.7％),黄狒狒(40.0％),橄榄狒狒(11.8％),白颊长臂猿(14.3％),婆罗洲猩猩(19.2％).对于不同的地区,郑州动物园的感染率为9.1％,石家庄动物园11.2％,武汉动物园7.6％,太原动物园13.6％,长沙野生动物园44.0％,北京动物园22.2％,上海动物园8.2％,上海野生动物园20.9％,以及河南,广西和广东猴场的感染率为2.9％.检测到的贾第虫为集聚体A(n=4)和集聚体B(n=118).对tpi基因序列的分析显示,集聚体A包括A1,A2和一个新的亚型.对tpi,gdh和bg基因的多位点分析共检测到18(7个已知和11个未报到),11(5已知和6个未报到)和9(2已知和7个未报到)的亚型在115,65和35个集聚体B分离株中.所有3个基因位点均成功扩增的32个集聚体B分离株中,共发现15个多位点基因亚型(MLGS),包括2已知和13未报到MLGs.集聚体B的串联序列的系统进化发育分析显示,这里发现的MLGs与来自人类,非人灵长类,兔和意大利和瑞典的豚鼠具有遗传差异.也进一步表明,环尾狐猴和松鼠猴分离的集聚体B与其他非人灵长类的可能存在差异.
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