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In this paper, a new stationary phase based on 1-allyl,3-methylimidazolium chloride ([AMIM]Cl)ionic liquid were synthesized by vinyltriethoxysilane modified silica and [AMIM]Cl.The capacity factors (k) of aromatic hydrocarbon decrease with the increase of methanol content in mobile phase, and the pH values of phosphate buffer solution.Thus, this phase represents anion-exchange and reverse phase mechanism.