【摘 要】
：
The calcium-activated chloride channels (CaCCs) are critical for many physiolo-gical functions.In cardiac tissues, CaCCs play important roles in the regulation of cardiac excitability.Although Anol (o
【机 构】
：
Department of Physiology,University of Qingdao Medical College,Qingdao,China
【出 处】
：
International Conference for Physiological Sciences 2012(201
论文部分内容阅读
The calcium-activated chloride channels (CaCCs) are critical for many physiolo-gical functions.In cardiac tissues, CaCCs play important roles in the regulation of cardiac excitability.Although Anol (or TMEM16A) has been identified as CaCCs in many tissues, the molecular identity of CaCCs in heart remains unknown.In this study, we examined the expression of Anol in rat heart by methods of reverse transcription polymerase chain reaction (RT-PCR) and Western blotting.RT-PCR shows the existence of Anol mRNA in heart tissue;while Anol protein expression were further confirmed by Western blotting, using HEK293 cells transfected with Ano1 cDNA as a positive control.The expression of heart Ano1 protein is higher in neonatal rats than that in adults.Furthermore, we noticed that phosphate-dylinositol 3-kinase (PI3K) inhibitor (LY294002) could up-regulate Ano1 expression in primarily cultured neonatal rats cardiomyocytes.Whole-cell patch-clamp studies showed that primarily cultured neonatal rat cardiomyocytes expressed CaCC currents.Conclusion: Our results strongly suggest that Ano1 protein exists in rat cardiomyocytes, and Ano1 expression may be regulated by PI3K pathway.
其他文献
Imbalance between angiotensin Ⅱ (Ang Ⅱ) and Ang 1-7 in the brain has been reported to be associated with hypertension.Exercise training (ExT) is capable of reducing blood pressure in hypertension.This
The peptide corresponding to the second extracellular loop ofangiotensin Ⅱ type 1 receptor (AT1R-ECⅡ)activating autoantibody (AT1-Ab), which was first detected in preeclampsia, can induce vascular smo
There are evidences suggesting the involvement of autoimmune mechanisms in the pathogenesis of dilated cardiomyopathy (DCM).Anti-β1/β2-adrenoceptor (AR) autoantibodies have been detected in the sera o
Our previous reseach show that Andrographolide (AP) has a protection on rats myocardial hypertrophy induced by isopropylarterenol (Iso), while its mechanisms about balance of vasoactive substance are
To observe the antioxidation and anti-inflammation effect of Genistein (Gen) on rats myocardial hypertrophy caused by isopropylarterenol (Iso).Methods: The myocardial hypertrophy model was formed thro
Abnormal arterial vasoconstriction is one of the major causes inducing post-flight orthostatic intolerance.It has been documented that Rho kinase (ROCK) could enhance the contraction of vascular smoot
Microgravity exposure may result in postflight orthostatic intolerance, which is associated with vascular remodeling.Nuclear factor κB (NF-κB) activation and increased inflammatory cytokines provide a
The aim of this study was to investigate whether decreased vasodilation in common carotid artery and abdominal aorta induced by hindlimb unloading (HU) is related with NADPH oxidase pathway.Arterial v
This study was designed to investigate the effects of 3-week simulated microgravity and intermittent artificial gravity on apoptosis of abdominal aorta smooth muscle cells (AASMCs) in rats.Hindlimb un
In vitro study has shown that activation of SK channels in the ventricular myocytes is responsible for the VF in failing ventricle.This study determined the role of SK channels in regulating VF in AMI