Haemaphysalis longicornis as a kind of obligatory blood-sucking ectoparasites, is one of the vector of pathogens such as bacteria, viruses, protozoa and rickettsia, affecting the health of humans and livestock, seriously restricting the development of animal husbandry in our country.Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix.