【摘 要】
：
已有数据显示Myostatin负调控肌肉干细胞的激活及自我更新过程,然而其分子机制并不明确。近年来的一些研究表明microRNAs在该过程中发挥重要作用。因此microRNAs可能参与Myostatin调控的干细胞功能。我们分别从Myostatin敲除小鼠及野生型小鼠中提取骨骼肌组织用并用微阵列分析法预测到受Myostatin负调控的miR-A,并且在Myostatin处理的C2C12细胞中也得到
【机 构】
：
中国医学科学院基础医学研究所生物化学与分子生物学重点实验室,北京,100005
论文部分内容阅读
已有数据显示Myostatin负调控肌肉干细胞的激活及自我更新过程,然而其分子机制并不明确。近年来的一些研究表明microRNAs在该过程中发挥重要作用。因此microRNAs可能参与Myostatin调控的干细胞功能。我们分别从Myostatin敲除小鼠及野生型小鼠中提取骨骼肌组织用并用微阵列分析法预测到受Myostatin负调控的miR-A,并且在Myostatin处理的C2C12细胞中也得到了证实。体内研究发现,与野生型小鼠相比在发育过程中miR-A转基因小鼠的干细胞数目及Pax7表达量均较低。同时我们在CTX肌肉损伤再生模型中发现Pax7及myoD表达量在miR-A转基因小鼠中均高于野生型小鼠,这些数据提示着miR-A可能在骨骼肌干细胞的激活及自我更新过程中有重要作用。结论：我们的数据显示miR-A受Myostatin调控并在骨骼肌干细胞的激活及自我更新过程中有着至关重要的功能。
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最新研究发现,内源性代谢中间产物在不同的生物学过程中都发挥着十分重要的调控功能,但目前已知具有这样功能的内源性代谢小分子却寥寥无几.我们通过系统发现鉴定了一个与骨骼肌发育相关的内源性代谢小分子X.实验表明内源性代谢小分子X剂量和时间依赖的促进C2C12和原代骨骼肌干细胞的增殖.同时,X可以协同IGF-1对肌细胞增殖的促进作用和拮抗Myostatin对骨骼肌干细胞增殖的抑制作用.同时,利用cardi
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