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Bombyx mori nucleopolyhedrovirus(BmNPV)is a major silkworm pathogen,causing substantial economic losses to the sericulture industry annually.We demonstrate a novel anti-BmNPV system expressing mature artificial microRNAs(amiRNAs)targeting the viral lef-11 gene.The mature amiRNAs inhibited the lef-11gene in silkworm BmN-SWU1 cells.