白洋淀水域同步硝化-反硝化菌的分离鉴定

来源 :第六届全国微生物资源学术暨国家微生物资源平台运行服务研讨会 | 被引量 : 0次 | 上传用户:kjtx123
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  白洋淀位于河北省中部保定地区,水域面积366平方公里,为华北平原最大的淡水湖,素有"华北明珠"之称。近年由于水体的富营养化,水质逐年恶化。同步硝化,反硝化(SND)是在微生物同一体系内同时具有硝化和反硝化功能,从而能够直接将氨态氮转化为无害的气体。本研究通过对白洋淀十个水区的水样、土样进行微生物的选择性培养,共分离得到异养硝化细菌183株,好氧反硝化菌1 17株,同步硝化反硝化菌64株。经16S rDNA测序及生理生化指标的初步鉴定,将异养硝化菌归属于15个属29个种,好氧反硝化菌归属于11属23种,同步硝化反硝化菌归属于9属19种。本研究为环境修复领域提供了优秀菌种资源,为硝化反硝化菌的在治理环境方面的应用提供了理论基础。
其他文献
The objective of this study was to investigate the endophytic microbial communities in Noni (Morinda citrifolia L.) whole plants, including the roots, branches, leaves, seeds and fruits of Noni.By usi
The objective of this study is to investigate the endophytic bacterial communities in Noni (Morinda citrifolia L.) seeds.By using high-throughput sequencing (HTS) technique, a research on the diversit
The current pigment applied in the industry is mainly synthesized by aromatic compounds such as benzene,toluene and naphthalene,but its security problems make it more and more limited.Compared with th
In the present study, we describe the isolation heterotrophic nitrifier with high ammonium and nitrite nitrogen removal ability from the aquaculture pond, and the nitrification performance of mixed cu
蜡样芽胞杆菌组是由多个亲缘关系密切的芽孢杆菌种的统称.尽管其在自然环境中分布广泛,且对人畜健康和国民经济发展具有重要的影响.但是,迄今为止,许多研究者对它们的分类地位和进化关系仍争论不休.同时,对于分离自不同海洋环境中的蜡样芽胞杆菌组菌株的多样性研究也鲜有报道.因此,本研究使用16S rRNA基因分析,基因组局部序列比对距离(GBDP)和数字化基因组杂交数值(dDDH)联合分析,多位点序列分析(M
For a long time, hydrogen peroxide and catalase had been widely utilized in the industrial sectors, but some sectors needed the catalase with high activity, alkali and thermo stabilization, which were
Natural dyes are widely used as sensitizer of dye-sensitized solar cells (DSSC) to make photoelectric conversion due to their low cost environment benign and safety.Diatoms are responsible for more th
Light is one of the most important factors for phototaxis growth in anoxygenic phototrophic bacteria.Rhodopseudomonas palustris has served as a model organism to elucidate spectrally different light-h
白蚁是自然界木质纤维素的主要分解者之一,白蚁和其肠道的多种微生物,在木质纤维素的降解过程中发挥重要作用.由于白蚁肠道微生物的不可培养性,微生物种类、数量及其具体作用都不清楚.本研究通过宏基因组16S rRNA测序,对高等培菌白蚁黄翅大白蚁中、后肠道的细菌多样性进行研究.结果发现后肠细菌数量和多样性明显高于中肠,后肠序列包含了16个门的细菌,而中肠只含有10个门的细菌.在中肠,多于96%的属于变形杆
利用细菌16S rRNA基因V3区PCR-DGGE分析研究了三种果蔬(苹果、萝卜、番茄)在家用冰箱贮藏前后表面的非培养和可培养微生物群落变化。研究结果表明苹果表面非培养和可培养细菌多样性显著低于萝卜、和番茄,可能与其在市售前的打蜡处理有关。贮藏前苹果表面的非培养细菌主要为Epilithonimonas和Pseudomonas属的菌株,冰箱贮藏7天后以Pseudomonas和Acinetobacte