Objective To clone the variable region genes of human anti-IL1RAP(IL-1 receptor accessory protein) monoclonal antibody(McAb) and construct IL1RAP chimeric antigen receptors(CARs).Methods The VH and VL DNA of IL1RAP single chain antibody were amplified by RACE and overlap extension PCR from total RNA that were extracted from 3H6E10、10D8A7 hybridoma and they were ligated into specific IL1RAP single-chain variable fragment(scFv).