Given the intensive and widespread application of the pesticide,buprofezin,its environmental residues potentially pose a problem;yet little is known about buprofezinskinetic and metabolic behaviors.In this study,a novel gram-positive strain,designated BF-5,isolated from aerobic activated sludge,was found to be capable of metabolizing buprofezin as its sole energy,carbon,and nitrogen source.Based on its physiological and biochemical characteristics,other aspects of its phenotype,and a phylogenetic analysis,strain BF-5 was identified as Bacillus sp.This study investigated the effect of culture conditions on bacterial growth and substrate degradation,such as pH,temperature,initial concentration,different nitrogen source,and additional nitrogen sources as co-substrates.