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Based on specific primers of Fusarium toxin gene, of 43 Fusarium graminearum clade strains which isolated from maize seeds of maize producing areas of north China were detected for the mycotoxin chemotypes.The results indicated that Fusarium graminearum clade, including the species of F.graminearum and F.asiaticum, were found to produce two kinds of mycotoxin chemotypes.F.graminearum was DON producer only, and F.asiaticum were DON and NIV producers.