【摘 要】
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Objective: To analysis and screen the key genes related to the characteristics of the elastic chondrocytes, the gene chip tech was used for the study of the differencial gene expression after the cart
【机 构】
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Pudong New Area Zhoupu Hospital,Plastic Surgery,Shanghai,201318
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Objective: To analysis and screen the key genes related to the characteristics of the elastic chondrocytes, the gene chip tech was used for the study of the differencial gene expression after the cartilage transposition.Methods: Healthy 8-week C57BL/6 mouses were randomly divided into three groups, one normal control group and two auricle cartilage transposition groups.The elastic cartilages were transplanted into the position of the ear cartilage defects and the subderma of the back.The normal ear cartilages and transposition tissues of the mouses were extracted for gene expression analysis.Results: Differencial expression genes were analysed,including gene related to oxidative stress (NDUFB1,NDUFB2, COX7A 1, UQCRQ), gene related to phosphorylation (NDUFA2,NDUFA3,COX7C,NDUFA1,COX17,ATP5H,COX6C,NDUFB6,COX6B1,COX5B,COL2A1 ,COL10A1, ALP, Runx2,ZHD-1 ,CPD), gene related to regeneration (NDUFB1,NDUFB2,COX7A1, UQCRQ, NDUFA2, NDUFA3, COX7C, NDUFA 1, ATP5H,COX6C,NDUFB6,COX6B1,COX5B,COL2A1,COL10A1,ALP,Runx2,ZHD-1,CPD), gene related to migration (COL2A1, COL10A1 ,ALP, Runx2, ZHD-1 ,CPD,NDUFB1, NDUFB2, COX7A1,UQCRQ,NDUFA2,NDUFA3, COX7C, NDUFA1, ATP5H, COX6C, NDUFB6,COX6B1,COX5B), gene related to ribosome (RPL3I,RPL37A, RPS19, RPL39, RPS11, PRS13, RPS25, RPL27,RPS23,RPLP2), gene related to RNA polymerase (POLR2I).Among those genes, 17 genes were up regulated and 6 genes were down regulated.Conclusions: There were significant differences in the gene expression profile of the transposition tissues and normal auricle elastic chondrocytes.The screened genes might be significant to the research for preserve of the characteristic of the elastic cartilage after the tissue transposition.
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