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Aim: B2 is a novel derivative of N6-(4-hydroxybenzyl) adenine riboside originally isolated from Gastrodia elata Blume.The ventrolateral preoptic nucleus (VLPO) is a group of neurons in the hypothalamus that release the inhibitory neurotransmitter γ-aminobutyric acid (GABA) during sleep.It plays an important role in sleepwake regulation.In this study, we examined the sedative and hypnotic effects of B2 and its influence on the VLPO in mice.