Aim: Insulin resistance plays an important role in the pathogenesis of diabetic cardiomyopathy.Berberine (BER) is a plant alkaloid which promotes hypoglycemia via increasing insulin sensitivity in peripheral tissues.Little is known of BBRs role in regulating glucose metabolism in heart.Methods: We examined the effect and mechanism of BER on glucose consumption and glucose uptake in insulin sensitive or insulin resistant rat H9c2 cardiomyocyte cells.H9c2 myoblast cells were differentiated into cardiomyocytes and incubated with insulin for 24 h to induce insulin resistance.