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Glyphosate-tolerant soybean (GTS) is a soybean cultivar introduced an exogenous gene which controls expression of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS).EPSPS is a key enzyme in secondary metabolism that would affect phenolic substances metabolism,and changes in metabolism of phenolic substances may occur comparing to its parent.In this research,phenolic substances were investigated in different fractions of soybean seed from GTS and its parent,and 3 conventional cultivars.