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Objective: To report our successful experience on sperm retrieval in ejaculate of nonmosaic Klinefelters syndrome (KS) patients with semen cytology examination.To describe clinical parameters of KS pa
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Objective: To report our successful experience on sperm retrieval in ejaculate of nonmosaic Klinefelters syndrome (KS) patients with semen cytology examination.To describe clinical parameters of KS patients with sperm compared to without sperm.Methods: A total of 151 patients were proven to suffer from Klinefelters syndrome by chromosomal analysis using G-banding.The semen samples were underwent the semen analysis and semen cytology examination.Correlation of patient clinical characteristics: age, testicular volume, serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T) levels were assessed for sperm recovery in ejaculate.Results: We succeeded in obtaining spermatozoa in 10 patients (10/151, 6.6%) with semen analysis.After being underwent semen cytology examination, a total of 32 patients (32/151, 21.2%) were found to have sperm or germ cell in ejaculate.The patients with successful sperm retrieval were significantly younger (27.1±3.7 yr) than those with failed attempt (28.9±34.2 yr).The mean serum testosterone level and the mean T/LH ratio of KS patients with successful sperm retrieval were significantly higher than men with sperm and in those without sperm (Testosterone: 3.2±2.1 ng/mL vs 2.7±1.5 ng/mL; T/LH ratio: 0.2.±0.3 vs 0.1±-0.1).Conclusion: semen cytology examination should be performed on identifying sperm and germ cell in ejaculate of KS patients, if no sperm is detected after traditional semen analysis.Serum testosterone level and T/LH ratio reveal an association between impaired Leydig-cell function and impaired spermatogenesis in KS males.We suggest KS patients should be accepted earlier diagnosis and treatment.
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