Nucleic acid extraction method based on silica-coated magnetic particles for RT-qPCR detection of pl

来源 :International Workshop on Viroids and Satellite RNAs(IWVdS)国 | 被引量 : 0次 | 上传用户：cronyGT

【摘要】

：

To develop a reliable and rapid nucleic acid extraction method and detecting technique for certification and quarantine programs on plant materials, the extract

【作者】

：

N.Sun X.-L.Zhao Q.Zhou C.-L.Deng W.-L.Yan Q.Xia

【机构】

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StateKeyLaboratoryofBioelectronics,SchoolofBiologicalScienceandMedicalEngineering,SoutheastUniversit

【出处】

：

International Workshop on Viroids and Satellite RNAs(IWVdS)国

【发表日期】

：

2013年8期

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To develop a reliable and rapid nucleic acid extraction method and detecting technique for certification and quarantine programs on plant materials, the extraction method based on silica-coated magnetic particles (SMPs method) and RT-qPCR assay were described.Arabis mosaic virus (ArMV), Lily symptomless virus (LSV), Hop stunt viroid (HSVd) and grape yellow speckle viroid 1 (GYSVd-1)was selected as research objects and the sequences including amplification regions of RT-qPCR were transcribed in vitro as standard RNA templates.The standard curves covered six or seven orders of magnitude with detection limits of 100 copies per each assay.

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Nucleic acid extraction method based on silica-coated magnetic particles for RT-qPCR detection of pl

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