【摘 要】
：
Obiective The objective of the present study was to compare the pathogenic properties of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) GD strain and its attenuated v
【机 构】
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China Institute of Veterinary Drug Control, Beijing, China;China Agricultural University, Beijing, C
【出 处】
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2013国际猪繁殖与呼吸综合征学术研讨会
论文部分内容阅读
Obiective The objective of the present study was to compare the pathogenic properties of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) GD strain and its attenuated vaccine strain GDr180 in piglets.Methods For this purpose,a total of thirty 35-day-old piglets were divided in 3 groups,each twelve piglets in group 1 and group 2 were separately intramuscularly inoculated with the GD strain of HPPRRSV and its attenuated vaccine strain GDr180,six piglets in group 3 served as uninoculated controls.Clinical signs and rectal temperatures were recorded daily.Each two piglets from group 1 and group 2,one piglets from group 3 were necropsied at 3,7,14,21,28 and 35 days postinoculation(DPI),respectively.The trachea,lung and pulmonary alveolar lavage fluid were collected for H.E.staining,immunohistochemical staining and scanning electron microscopic observation,besides,fluorescent quantitative RT-PCR (FQ-RT-PCR) were applied to determine the viral organic distribution.
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