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Objective：To investigate the feasibility of anatomical reconstruction of anterior cruciate ligament single bundle injury with hamstring tendons knot implant fixation. Methods From Feb 2002 to Feb 2005, 16 cases of anterior cruciate ligament posteriolateral bundle (PLB) reconstruction and 4 cases of anteriomedial btmdle(AMB)reconstruction were performed with hamstring tendons knot implant fixation. There were 10 cases of sports injuries, 6 cases of traffic accidents and 4 cases of daffy activity injuries. The ACL remnants were debrided arthroscopically and the uninjuried bundle was protected.Femoral tunnels were created according to the anatomic attachment of PLB (9:30 in the fight knee or 2:30 in the left) or AMB(11 in the fight knee or 1 in the left). Bottle-necked like femoral tunnel was created with a customized reamer. The diameter of tibial tunnel and inferior 1/3 of femoral tunnel were matched with the graft. After pretension, the hamstring tendon was knotted in the middle and then was introduced through the femoral tunnel into the tibial tunnel with the tendon knot press-fitted in the bottle-necked like femoral tunnel. Bis fixation and suture knotting on the bony bridge were completed in the tibial side. Result Patients were followed up for 6-8 months. Preoperative Lysholm score averaged 74.5 and postoperative score 94.2. Rulermetr examination showes that mean anterior tibial translation was less than 3mm and Lachmann test were negative postoperatively. Postoperative rotation was improved significantly. Conclusion：Anatomical reconstruction of anterior cruciate ligament single brindle (PLB/AMB)injury with hamstring tendons knot implant fixation has the advantages of biology fixation and superior compatibility.