论文部分内容阅读
Animals with conditional gene knockouts are valuable tools for studying mechanisms underlying cell and developmental biology.We have developed a conditional knockout strategy by spatiotemporally manipulating the expression of an RNA-guided DNA endonuclease,CRISPR-Cas9,in C.elegans somatic cell lineages.We showed that somatic CRISPR-Cas9 technology offers the highest possible speed to efficiently create conditional knockouts in various cell types at different developmental stages.