Conditional Knockouts Generated by Engineered Endonuclease Reveal Roles of Coronin in C.elegans Neur

来源 :第三届“模式生物与人类健康”发育遗传学全国学术研讨会 | 被引量 : 0次 | 上传用户：shaw1

【摘要】

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Animals with conditional gene knockouts are valuable tools for studying mechanisms underlying cell and developmental biology.We have developed a conditional kno

【作者】

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Guangshuo Ou

【机构】

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Tsinghua-PekingCenterforLifeSciences,SchoolofLifeSciences,TsinghuaUniversity,Beijing100084,China

【出处】

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第三届“模式生物与人类健康”发育遗传学全国学术研讨会

【发表日期】

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2014年7期

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Animals with conditional gene knockouts are valuable tools for studying mechanisms underlying cell and developmental biology.We have developed a conditional knockout strategy by spatiotemporally manipulating the expression of an RNA-guided DNA endonuclease,CRISPR-Cas9,in C.elegans somatic cell lineages.We showed that somatic CRISPR-Cas9 technology offers the highest possible speed to efficiently create conditional knockouts in various cell types at different developmental stages.

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Conditional Knockouts Generated by Engineered Endonuclease Reveal Roles of Coronin in C.elegans Neur

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