Loose-curd cauliflower (Brassica oleracea var.botrytis),a recently developed variety,has a long green flower peduncle,and good flavor.It is gaining popularity in China and Asia.B.oleracea var.botrytis is considered to be one of the most recalcitrant varieties of B.oleracea in terms of embryogenic response,although great effort has been devoted to refining techniques in this species.During more than 10 years of investigation,we developed a promising technique for highly efficient production of DH plants via microspore culture in loose-curd cauliflower.