1,3-propandiol is a very important chemical which is mainly used as building blocks for the synthesis of highly promising polyesters such as polytrimethylene terephthalate.We have previously constructed a genetically modified Klebsiella pneumoniae which can utilize glycerol as sole carbon source and produce 1,3-propanediol with the titer of higher than 90 g/L.A key factor affecting the production of 1,3-propanediol by the mutant Klebsiella pneumoniae is the precious control of feeding of glycerol.