Fusarium oxysporum is one of a very serious wilt pathogen in tree and fruit.The potential of using antagonistic as biocontrol agents to protect tree and fruit against it has long been recognized, Trichoderma sp.and Gliocladium sp.are widely used to control all kind of pathogen damage.This paper mainly using Trichoderma asperellum which from polar stem canker disease spot and Gliocladium roseum which from apple stem ring rot disease spot to control Fusarium oxysporum of wilt pathogen.The plate confrontation method was used.