论文部分内容阅读
本文介绍匀浆透析法制备山羊脾TF,经化学分析、紫外光谱扫描、高压液相层析、葡聚糖凝胶G—25层析和其分离组分的薄层层析、游离氨基酸分析等,初步说明与同法制备的人脾TF具有基本相似的组成成份。在体外非特异性活性反应中,羊脾TF显示出与人脾TF相似的活性,两者均能刺激人淋巴细胞内环核苷酸水平变化,使cGMP水平增高,cAMP水平不变或略下降,cGMP/cAMP比值显著增高,并能非常显著地促进37℃温育后人淋巴细胞与绵羊红细胞结合作用的恢复,提示羊脾TF作为免疫增强或触发剂,具有实际运用的可能性。
This article describes the preparation of goat spleen TF homogenate dialysis, chemical analysis, UV-Vis spectroscopy, high pressure liquid chromatography, Sephadex G-25 chromatography and its separation of components by TLC, free amino acid analysis , Preliminary description and the same method of human spleen TF has basically the same composition. In vitro nonspecific activity, sheep spleen TF showed similar activity to that of human spleen TF, both of which stimulated changes in nucleotide levels of human lymphocytes, increased cGMP levels, unchanged cAMP levels, or decreased slightly, cGMP / cAMP ratio was significantly increased, and can significantly promote the recovery of human lymphocytes and sheep erythrocytes after 37 ℃ incubation, suggesting that the spleen TF as an immune enhancement or trigger, with the possibility of practical use.