【摘 要】
：
Maize is one of the major food crops in the world,many researchers have focused on studying its agronomic traits related to the yield,especially the traits of the seeds.Here,we reported the molecular
【机 构】
：
Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China
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Maize is one of the major food crops in the world,many researchers have focused on studying its agronomic traits related to the yield,especially the traits of the seeds.Here,we reported the molecular and genetic characterization of small kernel 1(ski),a maize kernel size related recessive mutant which derived from a Mu tagging population.The sk1 locus was identified by the modified digestion-ligation-amplification (DLA) technology.Further complementation study on three additional independent Mu insertion alleles and nine transgenic lines confirmed that Sk1was responsible for the phenotype of sk1 mutant.BLASTP of SK1 shows that it belongs to 2H phosphodiesterase superfamily in maize and has conserved structure and function between plants,human,and yeast.Loss-function of Sk1 has a significant impact on the level and constitute of small noncoding RNA U6,a critical component of spliceosome in eukaryotes.A generalized retention of intron in transcriptome was detected according to the transcriptome sequencing data,totally 2063 genes contain the uncompleted spliced intron were detected in ski mutant.
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