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Objective:To set up the fat cell model of insulin resistance (IR) induced by high-glucose and insulin,and observe the effects of pumpkin polysaccharide (PP) on insulin resistance.Method:Fat cells were Primary cultured and joined with high-glucose (final 25mmol·L-1) and insulin (final 10nmol·L-1) to form the fat cells of insulin resistance.Pumpkin polysaccharide (PP) was added into the culture solution of fat cells of insulin resistance.