血球酶解工艺优化及其特性研究

来源 :中国畜牧兽医学会动物微生态学分会第十一次全国学术研讨会暨第五届会员代表大会 | 被引量 : 0次 | 上传用户:fishsun26
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  [目的]本试验旨在探索酸性蛋白酶水解鸭血球蛋白的最优工艺条件,开发一种新型肽粉,并研究其功能特质与体外抗氧化能力.[方法]通过单因素与正交试验调查酶用量、温度、pH、水解时间等因素对酶解反应的影响,确定最优工艺参数,研究其营养、功能与抗氧化特性.[结果]酸性蛋白酶酶解鸭血球蛋白的最优工艺参数为:酶用量(E/S) 6000 U/g,温度50℃,pH3.5,水解时间7h.采用该工艺条件,鸭血球蛋白水解度为25.10±0.65%,喷雾干燥血球肽粉蛋白含量为69.32±0.41%,限制性氨基酸异亮氨酸含量由0.44%提高至2.33%.与血球蛋白粉相比,血球肽粉呈乳白色,无刺鼻的金属味,具有良好的溶解性(>60%)、乳化稳定性和抗氧化活性.[结论]酸性蛋白酶可有效水解鸭血球蛋白,并获得蛋白含量高、氨基酸含量丰富且具有抗氧化活性的乳白色血球肽粉.
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