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Large-conductance calcium-activated K+ (BK-type, mSlol or mutations) channels, abundantly distributed in the excitable cells, response to the Ca2+-influx to regulate membrane potentials.However, it remains unknown how they react to the fast Ca2+-influx.Flash photolysis provides the most rapidly calcium release for detecting actions of Ca2+-binding proteins.In this study, our results revealed that the uncaged Ca2+ activated a voltage-and calcium-independent biphasic BK current with τf~0.2 ms and τs~10 ms, of which the fast component ratio Rf is voltage-dependent only.We further demonstrated that this occurred as the uncaged Ca2+ was not a constant.