This study aims to investigate the oxidative stress and hepatocellular injury induced by the oral administration of Cr3+ in chicken.Different doses of CrCl3 solutions (50% LD50, 25% LD50, and 12.5% LD50) and equivalent water were orally administered to Hylan Brown male chicken.Chicken liver samples were obtained every 2 weeks and measured the activities of antioxidant enzymes and the contents of glutathione, total antioxidant capacity, malondialdehyde, and hydrogen peroxide to indirectly evaluate the oxidative stress in chicken liver.Results indicated that the oral administration of Cr3+ at a high dose significantly increased (P<0.05) the MDA levels after 42 d of exposure.This observation was accompanied with the decrease in T-AOC, GSH and antioxidant enzymes activities.Similarity tendencies were observed when low and medium doses of Cr3+ long time were used.These statistics and histopathological analysis suggest that high-dose and long-term exposure of Cr3+ contributes to oxidative stress and hepatocellular injury.