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The rapid industrial development to cover the necessities of the huge population around the globe has serious effects on the environment.Highly populated countries have been facing the outbreak of emerging infectious diseases caused by pathogenic organisms.Virus,fungi,bacteria,and some pathogenic microorganisms are living extensively in the atmosphere with high reproduction rate,frightening the health of people and the environment.However,it is challenging to find some remedies against these bacteria to control permanent adhesive reaction.Recent decades,nanotechnology has attained remarkable attention to overcome some serious environmental issues such as energy conversion and biomedical application.Multifunctional nanomaterials have been proposed for the treatments of environments containing infectious pathogens.Particularly,metal and metal oxide-based disinfectants inorganic nanoparticles（NPs）are getting interest not only to improve the toxicity concerns related to organic materials but also to control the bacterial resistance against antibiotics.Nanocomposite has made potential attractions because of multifunctional usage and improved antimicrobial and photocatalytic activity better than single NPs.However,nanocomposite fabrications with low cost,control over size and stability issues are the obstacles for using these materials in biomedical field.In this work the simple hydrothermal method applied to prepare Ag-Ti O2nanocomposites and pure Ti O2 nanoparticles to improve antimicrobial activities of silk fibroin（SF）film.Moreover,splat shaped Ag-Ti O2 nanocomposites（NCs）are synthesized at gram scale for antimicrobial properties.Furthermore,these NCs are embedded with SF blending film.The formation of Ag-Ti O2 NCs and NCs based film are characterized by various techniques such as scanning electron microscopy（SEM）,energy dispersive spectroscopy（EDS）,X-ray diffraction（XRD）,Fourier transform infrared spectroscopy（FTIR）,ultra violet-visible spectroscopy（UV-VIS）and thermogravimetric analysis（TGA）applied to blended film to study the thermal behavior of blended film.Cell viabilities experiment has demonstrated good biocompatibility of the Ag-Ti O2 NCs.The antibacterial activity of the prepared NCs/film is tested against gram positive Staphylococcus aureus（S.Aureus）and gram negative Escherichia coli（E.coli）.The Ag-Ti O2 NCs and NCs based SF film have exhibited promising and superior antibacterial properties as compared to pure Ti O2 nanospheres which is confirmed by the bacterial growth and inhibition zone.The enhancement is attributed to the synergistic effect of hybrid nature of Ti O2 NPs in the presence of Ag contents.