Currently, several studies have been realized in order to erect Yarrowia lipolytica as afascinating microbial cell factory for many valuable products among which lipids, accumulatedin the form of Single Cell Oil (SCO), and terpenoids are considered as the main targets.However, Y.lipolytica suffers from a big industrial success because there is no specific surveyfocused on the development of adapted and pure fermentation platforms for these compounds.　　The present work was directed to the development of complete biochemically understandablebioprocesses for lipids and terpenoids production.In a first step, culture medium componentsscreening and filtering were undertaken in order to set up efficient and cost effective minimalculture media for lipid production from a wild type strain of Yarrowia lipolytica.The basicminimal culture medium (S2) designed yielded lipid content up to almost 35 ％ of the microbialdry cell weight.A set of fermentation strategies based on this minimal medium was developedand the lipid content was raised to 51％.The scale-up under different fermentation conditionsbased on S2 medium led to a maximum lipid content of 65 ％.The produced microbial oilsdisplayed interesting properties to be used as a feedstock for various applications.The minimalmedia and operable fermentation strategies devised in this study could enable fast; massive,viable and more economical production of single cell oils.Besides, the biotechnologicalproduction ofcarotenoids from Yarrowia lipolytica is an emerging scope that has not been fullyscrutinized, especially for what concerns cultivation conditions of newly generated engineeredstrains.By combining modelling and design of experiments, we screened chemicals forlycopene and astaxanthin production from engineered strains of Y.lipolytica.The modelling-assisted designed medium led to lycopene content of 4.35 mg/g DCW (76.62 mg/L) in flasksequivalent to 5.12 and 3.96 fold increase compared respectively to initial cultivation conditionsand the literatures-based designed medium.Lycopene concentrations of 126 and 242 mg/L wereachieved correspondingly from the modelling-assisted and the literatures-based designed mediain fed-batch cultivation mode.Transcriptional studies revealed the up-regulation ofheterologous genes in media designed according to flux balance analysis, thus implying theefficiency of the model predictions.In addition, a maximum astaxanthin content of 41 and 88μg/g DCW was obtained through flasks cultivations using the literature-based designed mediumand S2 medium, respectively.In a bioreactor fed-batch fermentation conditions, we obtained93.47 and 180 μg/g DCW ofastaxanthin content with astaxanthin production of 606 and 2109μg/L respectively in S2 and PM media.　　The novelty of this study is that it will permit the designing of pure, specific andindustrially cost-effective fermentation platforms for the manufacture of single cell oil (SCO)as a feedstock for different biotechnological applications, and particular terpenoids compounds.Furthermore, it will offer the possibility to produce particular yeast oils impregnated of valueadded pigments that will find application in cosmetics, pharmaceutical, nutraceutical and healthfields.Our study will also support upgraded lycopene, astaxantin and other terpenoidsproduction from existing or prospect bioengineered strains of Y.lipolytica.