论文部分内容阅读
目的考察研究莪术醇对HNE1鼻咽癌细胞荷瘤鼠的抑瘤作用。方法体外通过细胞划痕试验,考查莪术醇不同浓度(12.5μg/mL、25μg/mL、50μg/mL、100μg/mL)对HNE1鼻咽癌细胞迁移能力的影响,计算细胞迁移率(细胞迁移率(%)=(0 h划痕距离-12 h//24 h/48 h划痕距离)/0 h划痕距离×100%);体内采用HNE1鼻咽癌细胞接种裸鼠待长出实体瘤,将实体瘤移植裸鼠皮下建立HNE1鼻咽癌荷瘤鼠模型,用莪术醇分别给实验组裸鼠灌胃0.79 mg/10 g,对照组给灭菌蒸馏水,阳性组给予去氧氟尿苷,每日1次,连续30 d。每周测量体质量和肿瘤大小2次,第30天给药后处死小鼠,解剖称肿瘤质量,体积计算公式V=a×b×c×π/6,计算抑瘤率,评价莪术醇对HNE1鼻咽癌荷瘤鼠的抑制作用。结果与对照组比较,体外试验证明莪术醇100μg/mL对HNE1鼻咽癌细胞迁移性有统计学意义(P<0.05),体内试验证明莪术醇组对HNE1鼻咽癌细胞荷瘤鼠肿瘤的抑制作用明显,抑瘤率为34.04%,肿瘤质量与对照组比较有统计学意义(P<0.05),与对照组和去氧氟尿苷组比较,肝脏和脾脏系数均有统计学意义(P<0.05),且与去氧氟尿苷组比较,肝脏系数有统计学意义(P<0.05)。结论通过实验得出莪术醇能降低HNE1鼻咽癌细胞迁移能力并对荷瘤鼠肿瘤具有抑制作用,为莪术醇对肿瘤的抑制作用研究提供了实验基础。
Objective To investigate the antitumor effect of curcumol on tumor-bearing mice with HNE1 nasopharyngeal carcinoma. Methods The cell migration assay was used to evaluate the effect of curcumol at different concentrations (12.5μg / mL, 25μg / mL, 50μg / mL and 100μg / mL) on the migration ability of HNE1 NPC cells and the cell migration rate (%) = (0 h scratch distance -12 h / 24 h / 48 h scratch distance / 0 h scratch distance × 100%); in vivo HNE1 nasopharyngeal carcinoma cells inoculated nude mice to be solid tumors , The solid tumor-transplanted nude mice were established subcutaneously in HNE1 nasopharyngeal carcinoma model mice with curcumol were given to the experimental group nude mice gavage 0.79 mg / 10 g, the control group to sterile distilled water, positive group given doxifluridine , Once daily for 30 days. The body weight and tumor size were measured twice a week. After the administration on the 30th day, the mice were sacrificed and the tumor mass was anatomized. The volume calculation formula was V = a × b × c × π / 6. The inhibition rate of the curcumol HNE1 nasopharyngeal carcinoma tumor-bearing mice. Results Compared with the control group, curcumol 100 μg / mL had a significant effect on the migration of HNE1 nasopharyngeal carcinoma cells in vitro (P <0.05). In vivo experiments showed that curcumol inhibited the HNE1 nasopharyngeal carcinoma tumor-bearing mice The tumor inhibition rate was 34.04%. The tumor mass was significantly higher than that of the control group (P <0.05). Compared with the control group and the doxifluridine group, the coefficients of liver and spleen were statistically significant (P < 0.05). Compared with doxifluridine group, the liver coefficient was statistically significant (P <0.05). Conclusion Curcumol can reduce the migration ability of HNE1 nasopharyngeal carcinoma cells and inhibit the tumor of tumor-bearing mice, and provide experimental basis for the study of curcumol inhibiting the tumor.