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目的:在验证鼻咽癌(nasopharygycarcinoma,NPC)细胞CNE-1和CNE-2不同辐射抗拒性的基础上,探索微小RNA(miRNA)在CNE-1和CNE-2中的表达差异。方法:通过观察X线照射对CNE-1和CNE-2细胞克隆形成数目的影响,利用SigmaPlot软件进行分析及线性二次模型拟合存活曲线,比较CNE-1和CNE-2细胞剂量存活曲线及其生物学参数;采用Paraflo microfluidic microRNA芯片检测,用激光扫描器收集杂交的图像,经LOWESS滤器规范信号后分析数据差异,根据Targetscan3·1数据库资料(http:∥www·targetscan·org),预测CNE-1和CNE-2细胞microRNA差异表达与NPC放射敏感性差异的关系。结果:发现在CNE-1和CNE-2细胞中miRNA的差异表达,即与CNE-2细胞比,在检测的326个microRNA中,CNE-1细胞中有20个miRNA上调,13个miRNA下调,其中检测量的绝对值在2000以上且两者相差在3倍以上的miRNA有hsa-miR-152、hsa-miR-7、hsa-miR-205和hsa-miR-572;分析结果提示明显差异表达的miRNA与放疗敏感性密切相关。结论:不同辐射抗拒NPC细胞株CNE-1和CNE-2细胞的microRNA的表达有差异;差异表达的miRNA与放疗敏感有关。
OBJECTIVE: To explore the differential expression of microRNA (miRNA) in CNE-1 and CNE-2 based on the different radiation resistance of CNE-1 and CNE-2 in nasopharyngeal carcinoma (NPC) cells. Methods: The effect of X-ray irradiation on the number of CNE-1 and CNE-2 cell clones was observed. The SigmaPlot software and linear quadratic model were used to fit the survival curves. The survival curves of CNE-1 and CNE-2 cells were compared The biological parameters were determined by Paraflo microfluidic microRNA chip. The hybridization images were collected with a laser scanner. The data were analyzed by the LOWESS filter and the data were analyzed. Based on the Targetscan 3.1 database (http: // targetscan.org), the CNE 1 and CNE-2 cells microRNA differential expression and the relationship between NPC radiosensitivity. RESULTS: The differential expression of miRNAs in CNE-1 and CNE-2 cells was found that compared with CNE-2 cells, among the 326 microRNAs detected, 20 miRNAs were upregulated in CNE-1 cells and 13 miRNAs were down- Hsa-miR-7, hsa-miR-205 and hsa-miR-572 were detected in all the miRNAs whose absolute value was over 2000 and their difference was more than 3 times. The results showed that the miRNAs were obviously differentially expressed Of miRNAs are closely related to radiosensitivity. Conclusion: The expression of microRNAs in different radiation-resistant NPC cell lines CNE-1 and CNE-2 are different. The differentially expressed miRNAs are sensitive to radiotherapy.