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纤维板层型肝细胞癌是肝细胞癌中的一种独特亚型,主要发生在没有肝硬化的年轻人中。最近有报道纤维板层型肝细胞癌中存在重现性DNAJB1-PRKACA融合基因。为了确定该融合基因的特异性并开展常规有效的临床检测方法,作者对石蜡包埋组织进行RT-PCR和FISH检测PRKACA基因位点的重排情况,并通过原位杂交技术评估嵌合体转录物和野生型转录物RNA的情况。对106例原发性肝肿瘤通过RT-PCR进
Fibrolamellar hepatocellular carcinoma is a unique subtype of hepatocellular carcinoma that occurs mainly in young people without cirrhosis. Recently, it has been reported that there is a reproducible DNAJB1-PRKACA fusion gene in fibrolamellar hepatocellular carcinoma. In order to determine the specificity of the fusion gene and carry out routine and effective clinical detection methods, the authors performed RT-PCR and FISH to detect the rearrangement of the PRKACA gene locus in the paraffin-embedded tissue, and evaluated chimeric transcripts by in situ hybridization. And the case of wild-type transcript RNA. 106 cases of primary liver tumors were identified by RT-PCR