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石杉科植物因所含石杉碱甲(Huperzine A)对中老年痴呆等具有良好疗效,近年来倍受关注.利用AFLP分子标记对武夷山脉广布种长柄石杉[Huperzia serrata(Thunb.ex Murray)Trev.var.longipetiolata(Spring)H.M.Chang]7个居群112株个体进行遗传多样性和居群遗传结构分析.选用多态性高、分辨力强的8对选择性扩增引物组合共获得675个位点,其中多态位点比例为69.38%.居群内观测等位基因数(Na)为1.633,有效等位基因数(Ne)1.493;Nei’s基因多样性指数(He)与Shannon多态性信息指数(I)的平均值分别为0.272和0.392,多样性最高为地处武夷山脉中段的泰宁居群和建宁居群,最低为山脉北段的光泽居群.居群总基因多样性(Ht)为0.327 3,居群内基因多样性(Hs)为0.272 2,居群间的遗传分化系数(Gst)为0.168 1,表明居群内变异是长柄石杉遗传多样性的主要来源.由Gst估计,武夷山脉长柄石杉自然居群的基因流(Nm)为2.474 0.邻接树分析表明居群间遗传亲缘关系与地理位置相关.武夷山脉长柄石杉较高的遗传多样性和基因流水平表明其仍然具有相当的适应(生存)能力和进化潜力,这可能与其生物学(异交水平)、生态学特性及武夷山脉相对良好的生境条件有关.
Huperzine A has good curative effect on senile dementia and other dementia, and has drawn much attention in recent years.Using AFLP molecular marker, Huperzia serrata (Thunb. ex Murray) Trev.var.longipetiolata (Spring) HMChang The genetic diversity and population genetic structure of 112 individuals in seven populations were analyzed by using eight pairs of selective amplification primer combinations with high polymorphism and strong resolution A total of 675 loci were obtained, of which 69.38% were polymorphic loci.The number of observed alleles (Na) was 1.633 and the number of effective alleles (Ne) was 1.493 within the population.The Nei’s gene diversity index Shannon’s Polymorphism Information Index (I) averaged 0.272 and 0.392 respectively, with the highest diversity being the Taining population and the Jianning population located in the middle of the Wuyishan Range, with the lowest being the luster population in the northern segment of the range. The total gene diversity (Ht) was 0.327 3, the population genetic diversity (Hs) was 0.272 2, and the genetic differentiation coefficient (Gst) was 0.168 1 between populations, indicating that the intraspecific variation was genetic diversity According to Gst’s estimation, the gene flow (Nm) of the natural population of long-stemmed Huperzhany in Wuyi Mountains is 2.474 0. The adjacent tree Analysis showed that the genetic relationship between populations was related to the geographical location.The higher genetic diversity and gene flow of C. longifolia in Wuyishan Mountains indicated that it still possesses considerable adaptability (survival) and evolutionary potential, which may be related to its biology Out-crossing level), ecological characteristics and relative good habitat conditions of Wuyi Mountains.