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目的探讨胃癌中HER-2基因扩增与HER-2蛋白过表达的关系。方法运用免疫组化(IHC)和荧光原位杂交(FISH)技术,对手术切除的84例胃癌石蜡标本进行HER-2状态的检测。结果84例中,19例HER-2蛋白(+)(22.6%),其中HER-2基因扩增7例(8.3%);12例HER-2蛋白(/)(14.3%)中,HER-2基因扩增5例(6%),占高表达组的41.7%;72例HER-2蛋白(+/0)(85.8%)中,HER-2基因扩增2例(2.4%)。结论HER-2蛋白(/)与HER-2基因扩增密切相关,故这类患者可考虑先做IHC初步筛选,再做FISH确诊,从而为胃癌分子靶向治疗提供有价值的信息。
Objective To investigate the relationship between HER-2 gene amplification and HER-2 protein overexpression in gastric cancer. Methods The immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) were performed to detect the status of HER-2 in 84 cases of gastric cancer paraffin-embedded specimens. RESULTS: Of the 84 cases, HER-2 protein was found in 19 cases (22.6%) and HER-2 gene was amplified in 7 cases (8.3%). Among 12 cases of HER-2 protein (14.3% 2 gene amplification in 5 cases (6%), which accounted for 41.7% of the high expression group; 2 cases (2.4%) of HER-2 gene amplification in 72 cases of HER-2 protein (+ / 0) (85.8%). Conclusion The HER-2 protein (/) is closely related to the amplification of HER-2 gene. Therefore, these patients should consider IHC preliminary screening and then FISH diagnosis to provide valuable information for molecular targeted therapy of gastric cancer.