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目的测定和比较日本和国产不同工艺制柴苓汤颗粒剂中指标成分柴胡皂苷a的含量。方法采用HPLC,色谱柱为Kromasil C18(250 mm×4.6 mm,5μm);流动相为甲醇-水(75∶25);流速0.8 mL.min-1;柱温30℃;检测波长为210 nm。结果柴胡皂苷a线性范围为4.0~15.0μg,r=0.999 9,加样回收率为100.39%,RSD为0.80%。结论柴胡皂苷a的含量为日本津村柴苓汤颗粒剂高于国产仿日本工艺制柴苓汤颗粒剂高于国产习用工艺制柴苓汤颗粒剂。本方法简捷、方便、重复性好,准确度高,可用于柴苓汤颗粒剂的质量检测。
Objective To determine and compare the content of saikosaponin a, a component of the granules in different methods in Japan and in China. Methods HPLC was used. The chromatographic column was Kromasil C18 (250 mm×4.6 mm, 5 μm). The mobile phase was methanol-water (75∶25). The flow rate was 0.8 mL.min-1. The column temperature was 30°C. The detection wavelength was 210 nm. Results The linear range of saikosaponin a was 4.0~15.0μg, r=0.999 9, and the recovery was 100.39%. The RSD was 0.80%. Conclusion The content of saikosaponin a is higher than that of granules of Japanese Tsukura Chaiqin Decoction, which is higher than domestic granules of imitation Japanese Crafts, and that of Chaiqin Decoction. The method is simple, convenient, reproducible, and highly accurate, and can be used for quality detection of Chaiqin Tang granules.