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为研究人工合成甘蓝型油菜早期世代基因组及表达水平的遗传变异规律,本研究利用AFLP(扩增片段长度多态性)和cDNA-AFLP分子标记技术,以人工合成甘蓝型油菜的亲本、S0代、S3代植株为材料,用26对引物进行扩增。结果表明:S3世代在基因组水平共发生了6.33%的变异,其中发生在A基因组和C基因组上的变异条带分别占总扩增条带的1.68%和1.81%,不确定变异条带占总扩增条带的2.84%;S3世代在表达水平共发生了81.34%的变异,其中发生在A基因组和C基因组的变异条带分别占总扩增条带的16.36%和21.20%,30条变异条带来源于AC共有条带,占总扩增条带的6.91%;不确定变异条带占总扩增条带的36.87%。本研究结果说明人工合成甘蓝型油菜S3代基因组水平和表达水平均发生了一定程度的变异,其中表达水平的变异率较高,此外,基因组水平和表达水平的变异均倾向于发生在C基因组上。
In order to study the genetic variation of genomic and expression levels of artificially synthesized Brassica napus in early generations, AFLP (amplified fragment length polymorphism) and cDNA-AFLP molecular markers were used in this study. , S3 plants as materials, with 26 pairs of primers for amplification. The results showed that there was a total of 6.33% variation at the genome level in the S3 generation, of which the variation bands in A and C genomes accounted for 1.68% and 1.81% of the total amplification bands respectively, and the total number of uncertain bands The amplified bands were 2.84%. There were 81.34% variation in the S3 generation at the expression level, of which 16.36% and 21.20% of the total amplification bands occurred in the A and C genomes respectively and 30 variations The bands originated from AC common bands, accounting for 6.91% of the total amplified bands. The uncertain bands accounted for 36.87% of the total amplified bands. The results of this study showed that there was a certain degree of variation in the genomic level and expression level of the S3 generation of the synthetic B. napus rapeseed line with a high variation rate of the expression level. In addition, variation of the genome level and expression level tended to occur in the C genome .