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目的探讨孕妇胎盘组织中视黄酸诱导基因-Ⅰ(RIG-Ⅰ)、线粒体抗病毒信号蛋白(MAVS)的表达与乙型肝炎病毒(HBV)持续感染的关系。方法选取2015年5-10月深圳市福田区妇幼保健院产科收治的20例乙型肝炎病毒e抗原(HBe Ag)阳性和22例HBe Ag阴性慢性乙型肝炎孕妇为研究对象,另选取同期在该院孕检的正常孕妇22例作为对照组。采用实时荧光定量(RT-PCR)检测胎盘组织中RIG-Ⅰ和MAVS mRNA水平,采用RT-PCR、微粒子酶免疫分析法(MEIA)和酶联免疫吸附法(ELISA)分别检测其血清HBV DNA、HBe Ag及IFN-β水平。结果 HBe Ag阳性组孕妇胎盘组织中RIG-Ⅰ和MAVS mRNA水平明显低于正常对照组和HBe Ag阴性组,血清HBe Ag和HBV DNA水平明显高于正常对照组和HBe Ag阴性组,血清IFN-β水平显著低于正常对照组和HBe Ag阴性组,差异均有统计学意义(均P<0.01)。胎盘组织中RIG-Ⅰ、MAVS mRNA表达及外周血IFN-β水平与HBe Ag均呈负相关关系(r=-0.628,-0.675,-0.583,均P<0.05)。结论慢性乙型肝炎孕妇胎盘组织中RIG-Ⅰ、MAVS mRNA可能与HBV持续感染相关。HBe Ag对RIG-Ⅰ、MAVS和IFN-β表达起负调控作用。
Objective To investigate the relationship between the expression of retinoic acid-induced gene-Ⅰ (RIG-Ⅰ) and mitochondrial antiviral signal protein (MAVS) in placenta of pregnant women and the persistent infection of hepatitis B virus (HBV). Methods 20 pregnant women with HBe Ag positive and 22 HBe Ag negative chronic hepatitis B pregnant women admitted to Futian Maternal and Child Health Hospital from May to October in 2015 were enrolled in this study. 22 cases of normal pregnancy in pregnant women hospital as a control group. The levels of RIG-Ⅰ and MAVS mRNA in placenta were detected by real-time fluorescence quantitative PCR (RT-PCR). The serum HBV DNA, HBe Ag and IFN-β levels. Results The levels of RIG-I and MAVS mRNA in placenta of HBeAg-positive pregnant women were significantly lower than those in normal controls and HBeAg-negative pregnant women. The levels of serum HBeAg and HBV DNA in serum were significantly higher than those in normal controls and HBeAg- β levels were significantly lower than the normal control group and HBe Ag negative group, the difference was statistically significant (P <0.01). The expression of RIG-Ⅰ and MAVS mRNA and the level of IFN-βin the placenta were negatively correlated with HBeAg (r = -0.628, -0.675, -0.583, all P <0.05). Conclusion RIG-Ⅰ and MAVS mRNA in placenta of chronic hepatitis B may be associated with persistent HBV infection. HBe Ag negatively regulates the expression of RIG-I, MAVS and IFN-β.