应用~3H-dATP、~3H-dCTP标记不同的原癌基因探针c-abl、c-sis、3′-bcr及5′-bcr,对9例慢性粒细胞白血病(简称慢粒)患者骨髓或外周血中染色体9/22易位进行了原位杂交分析,结果显示,定位于9q34的c-abl转移至22号染色体,与5′-bcr发生重组;而c-sis定位于22q12.3～13.1,远离Ph易位断裂点,与慢粒发生无明显相关。就染色体原位杂交术在慢粒研究中的意义作了初步探讨。 The expression of c-abl, c-sis, 3’-bcr and 5’-bcr were detected by ~ 3H-dATP and ~ 3H-dCTP in bone marrow of 9 patients with chronic myeloid leukemia Or peripheral blood 9/22 translocation in situ hybridization analysis showed that located in 9q34 c-abl transferred to chromosome 22, and 5’-bcr recombinant; and c-sis located in 22q12.3 ~ 13.1, away from the Ph translocation breakpoint, and no significant correlation with the occurrence of chronic particles. The significance of chromosomal in situ hybridization in Cimicifuga was explored.