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【目的】观察蒿芩清胆汤及其化裁方对病毒性肺炎湿热证小鼠炎性细胞因子肿瘤坏死因子(TNF-α)、白细胞介素-6(IL-6)表达及肺组织流感病毒含量的影响。【方法】选用BALB/c小鼠,随机分为正常对照组、湿热证模型组、蒿芩清胆汤组(剂量为36.92 mg.kg-1.d-1)、化裁1组(剂量为30.39 mg.kg-1.d-1)、化裁2组(剂量为18.44 mg.kg-1.d-1)、化裁3组(剂量为32.73 mg.kg-1.d-1)、化裁4组(剂量为29.09 mg.kg-1.d-1);除正常对照组外,其他各组均采用高脂饲料+气候箱暴露(10 d)法复制湿热证模型,第11天接种H1N1病毒;取各组小鼠肺组织采用荧光定量聚合酶链反应法检测流感病毒mRNA、TNF-αmRNA、IL-6 mRNA表达水平。【结果】蒿芩清胆汤及各化裁方组均可降低肺组织流感病毒mRNA表达水平,与湿热证模型组比较差异有显著性意义(P<0.05或P<0.01);湿热证模型组肺组织TNF-αmRNA、IL-6 mRNA表达水平升高,与正常对照组比较差异具有显著性意义(P<0.01),各治疗组均可不同程度降低2种炎性细胞因子的表达水平,其中蒿芩清胆汤组,化裁2组(全方减竹茹、法夏、滑石、茯苓、甘草),化裁3组(全方减枳壳、陈皮)与湿热证模型组比较差异有显著性意义(P<0.05或P<0.01),而化裁1组(全方减青蒿、黄芩),化裁4组(全方减大青叶、板蓝根)与湿热模型组比较差异无显著性意义(P>0.05)。【结论】蒿芩清胆汤治疗流感病毒性肺炎湿热证的作用与其能抑制流感病毒复制,下调炎性细胞因子TNF-α、IL-6的基因表达水平有关,拆方研究结果表明蒿芩清胆汤全方效果最佳。
【Objective】 To observe the effects of Haoqin Qingdan Decoction and its recipe on the expression of inflammatory cytokines such as tumor necrosis factor (TNF-α), interleukin-6 (IL-6) and pulmonary influenza in mice with viral pneumonia The impact of virus content. 【Methods】 BALB / c mice were randomly divided into normal control group, damp-heat syndrome model group and Haoqin Qingdan Decoction group (dose was 36.92 mg.kg-1.d-1) 30.39 mg.kg-1.d-1), and the other two groups (dose of 18.44 mg.kg-1.d-1) and three doses of 32.73 mg.kg-1.d-1 The rats in each group were given damp-heat syndrome model by exposure to high-fat diet + climatic box for 10 days (except the normal control group) The H1N1 virus was inoculated. The lung tissue of each group was used to detect the mRNA expression of influenza virus mRNA, TNF-αmRNA and IL-6 by fluorescence quantitative polymerase chain reaction. 【Results】 Artemisia Qingqidan Decoction and all kinds of curative effect reduce the expression of influenza virus mRNA in lung tissue, which was significantly different from that of damp-heat syndrome model group (P <0.05 or P <0.01) The levels of TNF-αmRNA and IL-6 mRNA in lung tissue were significantly higher than those in normal control group (P <0.01). The levels of TNF-αmRNA and IL-6 mRNA in lung tissue were significantly decreased Artemisia Qingqian Decoction group, Huayu 2 group (Quanjuejuru, France Xia, talc, Poria, licorice), the cut 3 group (all the more Fructus aurantii, Citrus) compared with damp-heat syndrome model group was significantly different (P <0.05 or P <0.01). However, there was no significant difference between Huazu 1 group (Artemisia annua L. and Radix Scutellariae) and Huazhuo 4 group Significance (P> 0.05). 【Conclusion】 Artemisia Qingqian Decoction for the treatment of influenza virus damp-heat syndrome has the effect of inhibiting the replication of influenza virus and down-regulating the gene expression of inflammatory cytokines TNF-α and IL-6. Dan Tang best all-round effect.