论文部分内容阅读
目的:明确鼻咽癌(NPC)组织中人核迁移蛋白C(hNudC)的表达及其临床意义。方法:应用免疫组织化学方法和Western blot方法检测80例NPC和30例鼻咽黏膜慢性炎组织中hNudC的表达情况,并应用条带密度分析软件半定量分析Western blot条带密度。结果:在NPC中,hNudC阳性表达率为83.75%(67/80),明显高于鼻咽部黏膜慢性炎症组织23.33%(7/30)(P<0.05),hNudC蛋白的阳性表达率随着NPC临床分期的进展而增加(P<0.05)。结论:hNudC基因表达与NPC细胞恶性增殖密切相关,检测其表达对研究NPC的发生、发展具有一定临床意义。
Objective: To clarify the expression of hNudC in nasopharyngeal carcinoma (NPC) and its clinical significance. Methods: Immunohistochemistry and Western blot were used to detect the expression of hNudC in 80 cases of NPC and 30 cases of chronic nasopharyngeal mucosal inflammation. The density of Western blot was semi-quantitatively analyzed by using strip density analysis software. Results: The positive rate of hNudC in NPC was 83.75% (67/80), which was significantly higher than that in nasopharyngeal mucosal chronic inflammation tissue (23.33%, 7/30) (P <0.05). The positive rate of hNudC protein was The progress of NPC clinical stage increased (P <0.05). Conclusion: The expression of hNudC gene is closely related to the malignant proliferation of NPC cells. To detect the expression of hNudC gene is of clinical significance in the study of the occurrence and development of NPC.