免疫组织(细胞)化学染色中,各抗体间的浓度比至关重要。而在多层免疫反应中,最适浓度比的寻求颇为困难,且往往是决定成败优劣的关键。假如有四层抗体,每种抗体的稀释度分四级,其最少实验次数为4~4即256次。如何既不重复又无遗漏地进行256次排列组合试验;并且如何将如此庞大的工作量微缩使之微量快速高效地完成,迄今在国 Immunochemical (cell) chemical staining, the concentration of each antibody is crucial. In the multi-layer immune response, the search for the optimal concentration ratio is quite difficult, and it is often the key to determining the pros and cons of success or failure. If there are four layers of antibodies, the dilution of each antibody is divided into four levels, the minimum number of experiments for 4 to 4 that is 256 times. How to carry out 256 permutation and combination experiments without repetition and omission; and how to make such a tremendous workload miniaturized so that the micro-quantity can be quickly and efficiently completed. Up to now,