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本文介绍测定人血浆睾酮(T)的RIA(放射免疫分析)法,用混合酸酐法制备T-牛血清清蛋白结合物,以其为抗原制备T-抗血清,用1％活性碳分离免疫复合物与游离T,该方法的T的最小检测量为0.07pmol/L,批内系数分别为4.77％和1.64％,外加T标准品回收率为100.4％。测定158例正常人血浆T浓度,60岁以上男性明显低于60岁和批间变异以下者,女性更年期和绝经期明显低于育龄期,而月经各周期中T浓度无明显差异。
This article describes the RIA (radioimmunoassay) method for the determination of human plasma testosterone (T), the preparation of a T-bovine serum albumin conjugate by the mixed anhydride method, the preparation of T-antisera using it as an antigen, the immunocomplexation using 1% The minimum detectable amount of T for this method was 0.07 pmol / L, with an intra-assay coefficient of 4.77% and 1.64%, respectively. The recovery of T-labeled standard was 100.4%. 158 cases of normal plasma T concentration in men over the age of 60 was significantly lower than the 60-year-old and inter-batch variation below the menopause and menopausal women were significantly lower than the childbearing age, while the menstrual cycle in the T concentration was no significant difference.