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目的以全蝎为研究对象,比较水回流法、醇回流法、匀浆法、酶解法4种不同提取方法,优选最佳提取方法的工艺条件。方法以可溶性蛋白得率及总提取物得率为指标,确定最佳提取方法,并用小鼠抗惊厥药效验证该提取方法的可行性,进而采用正交试验优选出最佳工艺条件。结果从提取液蛋白得率及总提取物得率分析,匀浆提取明显优于其他3种方法;匀浆提取的最优工艺为加入12倍量的水,高速,匀浆提取2次。结论经小鼠抗惊厥药效验证,采用匀浆法对全蝎进行提取是可行的;验证试验表明,优选的匀浆提取工艺稳定可行。
Objective To take Scorpio as the research object and compare the four different extraction methods, ie water reflux method, alcohol reflux method, homogenization method and enzymolysis method, and optimize the optimum extraction conditions. Methods Soluble protein yield and total extract yield were used as indexes to determine the best extraction method. The anticonvulsant efficacy of the method was used to verify the feasibility of the extraction method. Then the optimum technological conditions were optimized by orthogonal test. Results The extraction rate of the extract protein and total extract yield, homogenate extraction was significantly better than the other three methods; homogenate extraction of the optimal process for the addition of 12 times the amount of water, high-speed, homogenate extraction 2 times. Conclusion It is feasible to extract the scorpion by homogenization after the anticonvulsant efficacy in mice is verified. The validation test shows that the optimized homogenate extraction process is stable and feasible.