建立了一种利用超快速高效液相色谱-三重四级杆串联质谱仪测定水中5种微囊藻毒素的方法。样品经滤膜过滤后,利用超快速高效液相色谱UFLC-20A XR进行分离,三重四级杆串联质谱LCMS-8040外标法进行定量分析。5种微囊藻毒素RR、YR、LR、LY、LF在0.1~50μg·L-1浓度范围内线性良好,定性检出限在0.0092μg·L-1~0.0743μg·L-1之间,定量限在0.0279μg·L-1~0.2253μg·L-1之间,加标回收率分别在85.4%~108.0%之间。该方法简便、快捷,适用于水中微囊藻毒素的测定。 A method for the determination of five microcystins in water using ultra-fast high performance liquid chromatography-triple quadrupole tandem mass spectrometry was established. The sample was filtered through a membrane filter and separated by ultra-fast high performance liquid chromatography (UFLC-20A) XR. Quantitative analysis was performed by triple quadrupole mass spectrometry LCMS-8040 external standard method. The linear ranges of RR, YR, LR, LY and LF of five microcystins were linear in the concentration range of 0.1-50 μg · L-1. The detection limits were between 0.0092 μg · L-1 and 0.0743 μg · L-1, The limits of quantification ranged from 0.0279μg · L-1 to 0.2253μg · L-1, with recoveries of 85.4% -108.0%. The method is simple and quick, and is suitable for the determination of microcystin in water.