论文部分内容阅读
目的牙周组织工程是修复因牙周炎导致的牙周组织缺损的研究热点。牙龈成纤维细胞(gingival fibroblasts,GFs)作为一种新的牙周组织工程的种子细胞,越来越多地受到人们的关注。研究通过GFs体外培养,诱导其向成骨细胞方向分化,探究其成骨分化能力。方法提取人GFs,传代培养后进行成骨诱导培养,并检测碱性磷酸酶及钙结节。结果与对照组比较,实验组人GFs经诱导后碱性磷酸酶增强,并产生较多的钙结节。结论人GFs经过体外培养及成骨诱导后,在牙周组织再生修复中有一定的应用价值。
Objective Periodontal tissue engineering is a hot research topic to repair periodontal tissue defects caused by periodontitis. Gingival fibroblasts (GFs), as a new periodontal tissue-engineered seed cells, are attracting more and more attention. The study of GFs in vitro culture induced osteoblasts to differentiate to explore its osteogenic differentiation. Methods Human GFs were isolated and subcultured for osteogenic induction culture. Alkaline phosphatase and calcium nodules were detected. Results Compared with the control group, the experimental group of human GFs increased after induction of alkaline phosphatase, and produce more calcium nodules. Conclusion Human GFs after in vitro culture and osteogenesis, periodontal tissue regeneration and repair has a certain value.