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目的:以牛粪为发酵基质,研究不同浓度Co2+对沼气发酵系统产气性能的影响,并利用分子生物学方法解析系统中的微生物群落。方法:测量不同Co2+添加量所对应的日产气量,累计产气量和甲烷含量;进行古菌16S rRNA基因克隆文库的构建及序列测定,以确定古菌多样性。结果:添加Co2+的实验组沼气累积产气量均高于对照组,0.1 mg·L-1·d-1的投加量对应的累积产气量最高,为1 815mL,高出对照组16%;各实验组对应的的古菌群落组成基本一致;甲烷微菌纲(Methanomicrobia)内的物种占绝对优势(总克隆的95%);另有少部分属于未确定的甲烷菌类群ArchⅠ(总克隆的1)%和泉古菌门(Crenarchaeota)(总克隆的4%)的物种。结论:Co2+可明显提高沼气的累积产气量,但添加需适量;Co2+对沼气发酵过程的微生物群落组成无明显影响。
OBJECTIVE: To study the effect of different concentrations of Co2 + on the gas production of biogas fermentation system using cow dung as fermentation substrate, and to analyze the microbial community by molecular biology method. Methods: The daily gas production, cumulative gas production and methane content were measured according to the amount of Co2 + added. The construction and sequencing of the archaeal 16S rRNA gene clone library were carried out to determine the archaeal diversity. Results: The accumulative gas production of biogas in the experimental group with Co2 + addition was higher than that of the control group. The highest cumulative gas production was 1 815 mL, which was the highest when the dosage of 0.1 mg · L-1 · d-1 was added, which was 16% higher than that of the control group The archaeal communities in the experimental group were basically the same in composition. The species in Methanomicrobia was predominant (95% of the total clones), and a small proportion of the unidentified methane group ArchⅠ 1)% and Crenarchaeota (4% of total clones) species. Conclusion: Co2 + can significantly increase the cumulative gas production of biogas, but the amount of biogas needed to be added in a proper amount. Co2 + has no significant effect on the biogas composition of biogas fermentation.