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目的研究类风湿关节炎(RA)患者外周血糖皮质激素诱导的肿瘤坏死因子受体家族相关基因(GITR)在CD4+CD25+T细胞中的表达及细胞因子IL-6、IL-17的浓度水平变化,探讨两者在RA发病中的作用。方法流式细胞术检测GITR在CD4+CD25+T细胞的阳性表达率,ELISA法检测血清中IL-6、IL-17的浓度水平并分析GITR阳性表达率与RA患者DAS28的关系。结果 RA组中的CD4+CD25+Treg细胞表面GITR阳性表达率(3.03±1.95)%明显低于健康对照组(7.68±2.01)%,RA组中IL-6,IL-17浓度水平明显高于健康对照组(P<0.05)。GITR阳性表达率与DAS28呈明显负相关。结论 RA患者GITR+CD4+CD25+T细胞阳性表达率低下,与RA发病及疾病的活动性有一定的相关性。
Objective To investigate the expression of peripheral blood glucocorticoid-induced tumor necrosis factor receptor family related genes (GITR) in CD4 + CD25 + T cells and the levels of IL-6 and IL-17 in patients with rheumatoid arthritis (RA) Change, to explore the role of the two in the pathogenesis of RA. Methods The positive rate of GITR in CD4 + CD25 + T cells was detected by flow cytometry. The levels of IL-6 and IL-17 in serum were detected by ELISA. The relationship between GITR positive rate and DAS28 was analyzed. Results The positive rate of GITR on the surface of CD4 + CD25 + Treg cells in RA group (3.03 ± 1.95%) was significantly lower than that in healthy controls (7.68 ± 2.01%). The levels of IL-6 and IL-17 in RA group were significantly higher than those in RA group Healthy control group (P <0.05). The positive rate of GITR was negatively correlated with DAS28. Conclusion The positive rate of GITR + CD4 + CD25 + T cells in RA patients is low, which is related to the incidence of RA and the activity of the disease.