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目的检测不同滋养细胞疾病组织中MMP-2/TIMP-2及VEGF的表达情况,观察比较它们的表达特点,分析与滋养细胞疾病发生、发展的相关性。方法收集30例正常绒毛;40例葡萄胎组织,30例手术切除的恶性葡萄胎组织,采用单克隆抗体免疫组化SP法对其染色,光镜下分别观察以上不同组织中MMP-2、TIMP-2、VEGF的阳性表达情况。结果(1)从正常绒毛→良性葡萄胎→恶性葡萄胎,MMP-2的阳性率有升高趋势,差异有统计学意义(P<0.05)。VEGF的表达与MMP-2正相关。(2)TIMP-2的阳性表达在滋养细胞疾病内部不同分组间两两比较,差异均无统计学意义(P>0.05)。(3)MMP-2/TIMP-2组合同时阴性表达在良性葡萄胎中占65%(26/40),在恶性葡萄胎中占16.67%(5/30),两者比较有显著性差异(P<0.01)。MMP-2阳性表达伴TIMP-2阴性表达在良性葡萄胎中占2.5%(1/40),在恶性葡萄胎中占56.67%(17/30),两者比较有显著性差异(P<0.01)。结论(1)MMP-2和VEGF共同参与了滋养细胞疾病的发生和发展。(2)MMP-2/TIMP-2两者联合检测对鉴别良、恶性滋养细胞疾病有重要价值。
Objective To detect the expression of MMP-2 / TIMP-2 and VEGF in different gestational trophoblastic disease tissues, and to observe their expression characteristics and to analyze the correlation with the occurrence and development of trophoblastic diseases. Methods Totally 30 cases of normal villi, 40 cases of hydatidiform mole and 30 cases of surgically resected malignant mole were collected and stained with monoclonal antibody immunohistochemical SP method. The expressions of MMP-2, TIMP -2, the positive expression of VEGF. Results (1) The positive rate of MMP-2 increased from normal villi → benign mole to malignant mole, the difference was statistically significant (P <0.05). The expression of VEGF is positively correlated with MMP-2. (2) The positive expression of TIMP-2 in trophoblastic disease within different groups between the two groups, the difference was not statistically significant (P> 0.05). (3) The negative expression of MMP-2 / TIMP-2 at the same time accounted for 65% (26/40) in benign hydatidiform moles and 16.67% (5/30) in malignant hydatidiform mole. There was a significant difference between the two P <0.01). The positive expression rate of MMP-2 and TIMP-2 were 2.5% (1/40) in benign hydatidiform mole and 56.67% (17/30) in malignant hydatidiform mole, with significant difference (P <0.01) ). Conclusion (1) MMP-2 and VEGF are involved in the occurrence and development of trophoblastic diseases. (2) The combined detection of MMP-2 / TIMP-2 has important value in the differential diagnosis of benign and malignant trophoblastic diseases.